FISH AND PRINS, A STRATEGY FOR RAPID CHROMOSOME SCREENING - APPLICATION TO THE ASSESSMENT OF ANEUPLOIDY IN HUMAN SPERM

Authors
PELLESTOR F QUENESSON I COIGNET L GIRARDET A ANDREO B LEFORT G CHARLIEU JP
Citation
F. Pellestor et al., FISH AND PRINS, A STRATEGY FOR RAPID CHROMOSOME SCREENING - APPLICATION TO THE ASSESSMENT OF ANEUPLOIDY IN HUMAN SPERM, Cytogenetics and cell genetics, 72(1), 1996, pp. 34-36
Citations number
13
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
72
Issue
1
Year of publication
1996
Pages
34 - 36
Database
ISI
SICI code
0301-0171(1996)72:1<34:FAPASF>2.0.ZU;2-L
Abstract
The co-utilization of FISH and PRINS techniques for in situ chromosome screening was tested on human sperm nuclei. We used a centromeric rep eat probe specific for chromosome 4 for FISH. PRINS reactions were per formed with a-satellite primers specific for either chromosome 9 or ch romosome 18. Double labeling was obtained and estimates of disomy rate s were carried out for the three chromosomes.