PHYSICAL MAPPING OF SOD1 TO BOVINE CHROMOSOME-1

Superoxide dismutase 1 (SOD1) was mapped to cattle chromosome 1q12-->q 14 by in situ methods. Both traditional in situ hybridization using tr itium and a new technique, direct in-situ single copy PCR (DISC-PCR), were used in two separate laboratories. Both human and bovine SOD1 clo nes were tritium labeled for radioactive in situ hybridization. A prim er pair based on the bovine SOD1 gene (Barendse et al., 1994b) was use d for the DISC-PCR procedure. The map location of SOD1 is close to col lagen 6A1. SOD1 is a potentially important type 1 anchor locus in the region where the gene for horns in cattle was recently mapped (Georges et al., 1993; Schmutz et al., 1995).