ENANTIOMERIC COMPOSITIONS OF MONOTERPENE HYDROCARBONS IN DIFFERENT TISSUES OF 4 INDIVIDUALS OF PINUS-SYLVESTRIS

The relative amounts of volatiles, mainly monoterpene hydrocarbons, we re determined in eight different tissues of each of four individuals o f Pinus sylvestris. The four trees represented widely different monote rpene compositions. Two-dimensional gas chromatography, using columns with stationary phases containing functionalized cyclodextrins, allowe d the determination of the enantiomeric compositions of seven major ch iral monoterpene hydrocarbons. Large differences in the relative amoun ts of the monoterpene hydrocarbons as well as in their enantiomeric co mpositions were found both within and between individuals. The extreme ly large relative amounts of (+)-3-carene or (-)-limonene found in the samples of 1-year-old phloem and bark of branches and in the shoots o f some of the trees were not found in the xylem of the trunk or root o r in the needles of the same trees. Only the (+)-enantiomer of 3-caren e was detected. The (-)-enantiomers of beta-pinene, sabinene and beta- phellandrene dominated over the corresponding (+)-enantiomers in all s amples.