HIGH-RESOLUTION THIN-LAYER CHROMATOGRAPHY OF GANGLIOSIDES

In this review an updated overview of current improvements on thin-lay er chromatography (TLC) of gangliosides over the past decade is provid ed. Basic general techniques and special advice is given for successfu l separation of glycosphingolipids. New approaches concerning continuo us and multiple development, and several preparative TLC methods are a lso included. Emphasis is placed on TLC immunostaining and related tec hniques, i.e. practical applications of carbohydrate-specific antibodi es, toxins and bacteria, viruses, lectins and eukaryotic cells. Thus, this review on ganglioside TLC summarizes its power as an analytical t ool for a wide range of purposes.