SUPERCRITICAL-FLUID CHROMATOGRAPHY OF CARBOHYDRATES

Supercritical fluid chromatography (SFC) is a relatively new technique applied to polar solutes such as carbohydrates about 10 years ago. Th e developments in the SFC of carbohydrates are summarized and a compar ison between capillary and packed column SFC is presented. High-effici ency capillary columns are suitable only for derivatized carbohydrates since various packed columns are well adapted for non derivatized mon o-, di- and trisaccharides and provide complementary selectivities.