RECENT PROGRESS IN CARBOHYDRATE SEPARATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY BASED ON HYDROPHILIC INTERACTION

This article surveys recent developments in the separation and analysi s of carbohydrates by high-performance liquid chromatography, in adsor ption or partition modes, on polar sorbents with less polar eluents, a technique that is now termed hydrophilic interaction chromatography. A variety of chromatographic methods are included under this generic h eading, the most important being adsorption chromatography on silica a nd partition chromatography on silica-based sorbents bearing bonded po lar phases. Examples are given of the applications of these stationary phases, as well as the newer polymer-based polar sorbents, in high-pe rformance liquid chromatography of carbohydrates and their derivatives .