COMPARISON OF SEPARATION MODES OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE ANALYSIS OF GLYCOPROTEIN-DERIVED AND PROTEOGLYCAN-DERIVED OLIGOSACCHARIDES

Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released fro m proteoglycans and of fluorescently labelled oligosaccharide derivati ves for high-sensitivity detection. Sulphated oligosaccharides show go od retention and separation behaviour on PGC-HPLC, and compared to ani on-exchange or reversed-phase ion-pair chromatography the chromatograp hy is carried out in the absence of salt. Due to their poor retention on PGC-HPLC the analysis of single uronic acids has been optimised wit h high pH anion-exchange chromatography. Fluorescent labelled derivati ves formed by reductive amination of neutral oligosaccharides with 2-a minobenzamide have been chromatographed on PGC-HPLC and by BioGel P4 g el filtration.