IMMUNOHISTOCHEMICAL DEMONSTRATION OF ADENOHYPOPHYSEAL HORMONES DURINGPOSTNATAL ONTOGENY IN THE GRAY SHORT-TAILED OPOSSUM, MONODELPHIS-DOMESTICA (MARSUPIALIA)

Antibodies against human FSH, LH, TSH, ACTH, GH, PRL, and MSH were app lied to paraffin sections of newborn (<1) and pouch-young individuals with the age of 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 13, 14 days, respectiv ely (D1, D2,...). In the pouch-young, the antibody reactions occurred only in the pars distalis of the adenohypophysis. The D<1 individuals included, the reactions were strong for ACTH, moderate/strong for GH, and moderate for MSH. Reactivity for TSH was recorded first in D2 spec imens, for LH in D3 animals, for FSH and PRL in D8 animals. There was a remarkable regional distribution of reactive cells within the pars d istalis: reactions for ACTH were localized mainly in the rostral area, those for GH were most prominent caudally. The chronological sequence of appearance of the specific hormone-producing cells in the pouch-yo ung generally is in accordance with the characteristic pattern known i n other mammalian species. The late onset of differentiation of some c ell types indicates the research possibilities that postnatal Monodelp his domestica can offer in the field of ontogenetic studies on the mam malian endocrine system.