SEPARATION OF H7-RESISTANT PROTEIN-KINASE-C ISOFORMS FROM GONADOTROPH-DERIVED ALPHA-T3-1 CELLS

Certain physiological events in anterior pituitary tissue are regulate d by protein kinase C that is relatively resistant to the inhibitor. H 7. In the present studies we have shown that two H7-resistant protein kinase C activities may be isolated from (pituitary) gonadotroph-deriv ed alpha T3-1 cells using hydroxyapatite chromatography. These activit ies have the characteristics of PKC zeta and an apparently novel PF;C isoform. The availability of clonal alpha T3-1 cells will facilitate i nvestigations of the pituitary events. (C) 1996 Academic Press, Inc.