SPECIFIC INTERACTIONS OF ANTIRETROVIRALY ACTIVE-DRUG HYPERICIN WITH DNA AS STUDIED BY SURFACE-ENHANCED RESONANCE RAMAN-SPECTROSCOPY

Citation
S. Sanchezcortes et al., SPECIFIC INTERACTIONS OF ANTIRETROVIRALY ACTIVE-DRUG HYPERICIN WITH DNA AS STUDIED BY SURFACE-ENHANCED RESONANCE RAMAN-SPECTROSCOPY, Journal of physical chemistry, 100(5), 1996, pp. 1938-1944
Citations number
55
Categorie Soggetti
Chemistry Physical
ISSN journal
00223654
Volume
100
Issue
5
Year of publication
1996
Pages
1938 - 1944
Database
ISI
SICI code
0022-3654(1996)100:5<1938:SIOAAH>2.0.ZU;2-G
Abstract
Surface-enhanced resonance Raman spectroscopy was employed to study th e interaction of hypericin with DNA and its model molecules poly(rA) a nd poly(rG). The obtained results clearly demonstrate that hypericin a ctually interacts with DNA and that terminal hydroxyl and carbonyl gro ups are directly implicated in this interaction. A detailed study of D NA structural marker bands in the 600-850 cm(-1) region reveals that g uanine plays a crucial role in this interaction. This deduction was co nfirmed by studying the interaction of hypericin with poly(rG) complex , in which evident changes concerning both hydroxyl and carbonyl group s bands of hypericin and guanine ring breathing band of the polynucleo tide can be observed. In view of these results, a model for the hyperi cin-DNA interaction is proposed.