S. Sanchezcortes et al., SPECIFIC INTERACTIONS OF ANTIRETROVIRALY ACTIVE-DRUG HYPERICIN WITH DNA AS STUDIED BY SURFACE-ENHANCED RESONANCE RAMAN-SPECTROSCOPY, Journal of physical chemistry, 100(5), 1996, pp. 1938-1944
Surface-enhanced resonance Raman spectroscopy was employed to study th
e interaction of hypericin with DNA and its model molecules poly(rA) a
nd poly(rG). The obtained results clearly demonstrate that hypericin a
ctually interacts with DNA and that terminal hydroxyl and carbonyl gro
ups are directly implicated in this interaction. A detailed study of D
NA structural marker bands in the 600-850 cm(-1) region reveals that g
uanine plays a crucial role in this interaction. This deduction was co
nfirmed by studying the interaction of hypericin with poly(rG) complex
, in which evident changes concerning both hydroxyl and carbonyl group
s bands of hypericin and guanine ring breathing band of the polynucleo
tide can be observed. In view of these results, a model for the hyperi
cin-DNA interaction is proposed.