If. Shayakhmetov et Fm. Shakirova, SOMATIC EMBRYOGENESIS IN WHEAT CELL-SUSPENSION CULTURES IN THE PRESENCE OF ABSCISIC-ACID, Russian journal of plant physiology, 43(1), 1996, pp. 88-90
The effects of 7.6 mu M ABA added to Murashige and Skoog nutrient medi
um on the growth rate and the initiation of embryogenic cell aggregate
s were studied on suspension cultures of spring hard wheat (Triticum d
urum L., cv. Khar'kovskaya 46). For 28 days of culturing, ABA substant
ially decreased the total cell volume, lowered the content of endogeno
us IAA three times, and prevented early formation of root primordia kn
own to hinder further embryogenesis. ABA is suggested to facilitate ma
turation of embryoids by shifting the hormonal balance in cultured cel
ls.