DENATURING GRADIENT GEL-ELECTROPHORESIS PROFILES OF 16S RIBOSOMAL-RNA-DEFINED POPULATIONS INHABITING A HOT-SPRING MICROBIAL MAT COMMUNITY

Citation
Mj. Ferris et al., DENATURING GRADIENT GEL-ELECTROPHORESIS PROFILES OF 16S RIBOSOMAL-RNA-DEFINED POPULATIONS INHABITING A HOT-SPRING MICROBIAL MAT COMMUNITY, Applied and environmental microbiology, 62(2), 1996, pp. 340-346
Citations number
36
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
62
Issue
2
Year of publication
1996
Pages
340 - 346
Database
ISI
SICI code
0099-2240(1996)62:2<340:DGGPO1>2.0.ZU;2-Y
Abstract
Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rR NA gene segments was used to profile microbial populations inhabiting different temperature regions in the microbial mat community of Octopu s Spring, Yellowstone National Park. DGGE allowed a rapid evaluation o f the distributions of amplifiable sequence types. Profiles were essen tially identical within regions of the mat defined by one temperature range but varied between sites with different temperature ranges, Indi vidual DGGE bands were sequenced, and the sequences were compared with those previously obtained from the mat by cloning and from cultivated Octopus Spring isolates. Two known cyanobacterial populations and one known green nonsulfur bacterium-like population were detected by DGGE , as were many new cyanobacterial and green nonsulfur and green sulfur bacterium-like populations and a novel bacterial population of uncert ain phylogenetic affiliation. The distributions of several cyanobacter ial populations compared favorably with results obtained previously by oligonucleotide probe analyses and suggest that adaptation to tempera ture has occurred among cyanobacteria which are phylogenetically very similar.