EFFECTS OF CISPLATIN ON CELL-CYCLE KINETICS, MORPHOLOGICAL CHANGE, AND CLEAVAGE PATTERN OF DNA IN 2 HUMAN OVARIAN-CARCINOMA CELL-LINES

We evaluated the effects of cisplatin on the cell kinetics, cytomorpho logical changes, and cleavage patterns of DNA in two lines of human ov arian carcinoma cells. KF-1 cells displayed a cell cycle arrest in the G(2)M phase, while HMG cells displayed a transient cell accumulation in the S phase, without obvious G(2)M arrest. Morphological changes ch aracterized by condensation and fragmentation of chromatin, and DNA cl eavage by oligonucleosome-sized DNA fragments were observed in the HMG cells but not in the KF-1 cells. The pattern of cell death in HMG cel ls was considered to be apoptosis, but that of KF-1 cells necrosis. Th ese findings showed the different mechanisms of anti-tumor effect of c isplatin on human ovarian carcinoma cell lines, including cell kinetic s and pattern of cell death.