APPLICATION OF THE DIRECT COLONY TLC FOR IDENTIFICATION OF PHYTOPATHOGENIC BACTERIA .3. DISTINCTION OF THE PSEUDOMONADS IN THE RIBOSOMAL-RNA-HOMOLOGY GROUP-II (BURKHOLDERIA SPP)

The comparison of chromatographic profiles of pseudomonads in the rRNA homology-group II (Burkholderia gen. nov.) was conducted by using the direct colony TLC method. The chromatograms of Pseudomonas cepacia, P . caryophylli, P. glumae, P. gladioli, P. plantarii, P. andropogonis, P. solanacearum and P. rubrisubalbicans resembled roughly each other. However, the profiles of three spots which were designated as S1, S2 a nd S3 were distinct at species level. The chromatograms of P. andropog onis, P. solanacearum and P. rubrisubalbicans were characteristic. Esp ecially, the profile of P. rubrisubalbicans was quite unique in these pseudomonads. So far as the chromatograms for aminolipids concerned, s ome similarities were observed between P. andropogonis and P. solanace arum. However, P. andropogonis was distinguishable with the existence of single spot which is detectable at Rf 0.83 by spraying of the chrom ium-containing 55% sulfuric acid and heating at 110 degrees C. The use fulness of the direct colony TLC method for rapid identification of ph ytopathogenic bacteria at species level was repeatedly verified.