A SIMPLE DIRECT ENZYME-IMMUNOASSAY OF ANTIBODIES BASED ON THE DIFFERENCES IN-DIFFUSION RATES IN A GEL OF A SYNTHETIC ANTIGEN AND OF ITS COMPLEX WITH ANTIBODIES
An. Semenov et Ee. Efremov, A SIMPLE DIRECT ENZYME-IMMUNOASSAY OF ANTIBODIES BASED ON THE DIFFERENCES IN-DIFFUSION RATES IN A GEL OF A SYNTHETIC ANTIGEN AND OF ITS COMPLEX WITH ANTIBODIES, Applied biochemistry and biotechnology, 56(1), 1996, pp. 73-78
A simple direct enzyme immunoassay for semiquantitative detection of a
ntibodies is suggested. It is based on the difference in diffusion rat
es in a gel for a synthetic low-mol-wt antigen and of its complexes wi
th antibodies to be detected. Sensitivity and specificity of the devel
oped assay are equal to an ELISA method. The assay has been tested wit
h antibodies against HIV protein gp41 in rabbit serum. Possible applic
ations and limitations of the method are discussed.