A SIMPLE DIRECT ENZYME-IMMUNOASSAY OF ANTIBODIES BASED ON THE DIFFERENCES IN-DIFFUSION RATES IN A GEL OF A SYNTHETIC ANTIGEN AND OF ITS COMPLEX WITH ANTIBODIES

Authors
SEMENOV AN EFREMOV EE
Citation
An. Semenov et Ee. Efremov, A SIMPLE DIRECT ENZYME-IMMUNOASSAY OF ANTIBODIES BASED ON THE DIFFERENCES IN-DIFFUSION RATES IN A GEL OF A SYNTHETIC ANTIGEN AND OF ITS COMPLEX WITH ANTIBODIES, Applied biochemistry and biotechnology, 56(1), 1996, pp. 73-78
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
56
Issue
1
Year of publication
1996
Pages
73 - 78
Database
ISI
SICI code
0273-2289(1996)56:1<73:ASDEOA>2.0.ZU;2-T
Abstract
A simple direct enzyme immunoassay for semiquantitative detection of a ntibodies is suggested. It is based on the difference in diffusion rat es in a gel for a synthetic low-mol-wt antigen and of its complexes wi th antibodies to be detected. Sensitivity and specificity of the devel oped assay are equal to an ELISA method. The assay has been tested wit h antibodies against HIV protein gp41 in rabbit serum. Possible applic ations and limitations of the method are discussed.