PHYSICAL MAP OF ANAGRAPHA-FALCIFERA MULTINUCLEOCAPSID NUCLEAR POLYHEDROSIS-VIRUS

A physical map of Anagrapha falcifera multinucleocapsid nuclear polyhe drosis virus (AfMNPV) DNA was constructed for restriction endonuclease s EcoRI, HindIII, PstI and XhoI. The genome size was estimated to be 1 30 kbp. Ordering of the restriction fragments was accomplished by cros s-blot hybridization, double digestion and DNA-DNA hybridization. The polyhedrin gene and homologous repeat (hr) regions were located by hyb ridization to the Autographa californica MNPV (AcMNPV) polyhedrin gene and hr4, respectively. Restriction pattern comparison and Southern bl ot analysis suggest that AfMNPV is closely related to AcMNPV.