EXCRETION OF OXYTETRACYCLINE RESIDUES BY MILK OF COWS WITH CLINICAL MASTITIS DURING LACTATION PERIOD

The objective of this study was to determine the oxytetracycline resid ues in milk from cows with clinical mastitis dosed with two extra-labe l routes of oxytetracycline administration not only during antibiotic' s treatment (5 days), but also six days after treatment by use of a li quid chromatography method of testing with a detection limit of 20 ppb . Both groups of animals were treated once daily for five milkings at 24-hour intervals following morning milkings. Composite milk samples ( equal volumes of foremilk from each quarter) were collected during mor ning and afternoon milkings, mixed together (1 : 1), and stored until analyzed. Milk samples were analyzed just before the first treatment ( 0 hour) and ten times at 24-hour intervals. Residue studies in milk co ws indicate that oxytetracycline passes into milk. Residues in milk we re higher for the cows receiving oxytetracycline by intramammary route (Tab. I) than for the cows receiving oxytetracycline intramuscularly (Tab. II). The highest mean data were 195.68 mg/kg after intramammary infusion (Fig. 2) and 2.74 mg/kg after intramuscular injection (Fig. 3 ) on the 5th day of the treatment beginning. The analysis data showed that oxytetracycline persisted in milk for as long as two days after b oth treatments at the concentration 0.03 mg/kg versus 0.02 mg/kg, resp ectively. No residues were detected in milk of any animal from the 4th day of the cessation of the therapy (Fig. 1) as detected by the HPLC method.