S. Gunasekaran et al., ACINAR-CELLS FREE PURE ISLETS FOR TRANSPLANTATION FROM INDIAN BONNET MONKEYS, Indian journal of medical research. Section B: Biomedical research other than infectious diseases, 98, 1993, pp. 293-296
Pancreatic islets, completely free of acinar cells were isolated from
the Indian bonnet monkey Macaca radiata radiata for transplantation st
udies. The monkey pancreas was inflated with Hank's solution containin
g collagenase (5 mg/ml). After incubation for 25 min at 37 degrees C t
he friable pancreas was teased apart. The fragments were aspirated wit
h a 20 ml disposable syringe (without needle) twice and ejected. After
the third aspiration, the homogenate was poured over nylon mesh. Isle
ts were separated from the filtrate by centrifugation in Ficoll gradie
nts and then hand picked. Histological examination revealed that the i
slets were completely free of acinar cells. On exposure to glucose, th
e islets responded normally with increased insulin release. They retai
ned their structural integrity after transplantation under the renal c
apsule of normal rats treated with cyclosporine. The deleterious effec
t of exocrine cells after transplantation will be completely eliminate
d by this islet preparation.