R. Lowe et al., SIMULTANEOUS SEPARATION OF THE BETA-LACTOGLOBULIN-A, BETA-LACTOGLOBULIN-B AND BETA-LACTOGLOBULIN-C VARIANTS USING POLYACRYLAMIDE-GEL ELECTROPHORESIS, Milchwissenschaft, 50(12), 1995, pp. 663-666
A method for the simultaneous separation of the A, B and C variants of
beta-Lg was developed using polyacrylamide slab gel electrophoresis.
A resolving gel of 6% acrylamide (2.6% Bis acrylamide) at pH 8.5 in a
sodium berate buffer system was used. The inclusion of a stacking gel
of 4% acrylamide (2.6% Bis) at pH 6.8, although not essential improved
the separation. Because the casein proteins migrated in the same posi
tion as the beta-Lg C variant, it was necessary to remove casein from
milk samples by acid precipitation and centrifugation. Running conditi
ons involved high voltages (210 V) for short periods in order to focus
the proteins into a sharp band and to migrate the proteins rapidly in
to the resolving gel, followed by low voltages to achieve separation o
f the beta-Lg variants.