RAPID METHOD FOR ISOLATION OF HIGH-MOLECULAR-WEIGHT PLASMIDS SUITABLEFOR DIGESTION WITH RESTRICTION ENDONUCLEASES

The described method is a modification of the hot alkaline method (Kad o and Liu, 1981). The modification lies in replacement of SDS with Tri ton X-100. The DNA purified by this method was readily digested with r estriction endonucleases for a variety of applications, e. g. restrict ion analysis of plasmid DNA in epidemiological studies, restriction ma pping and cloning of high molecular weight plasmids, and for identific ation of transpozon mutants.