PURIFICATION OF SUBUNIT-B OF SHIGA TOXIN USING A SYNTHETIC TRISACCHARIDE-BASED AFFINITY MATRIX

Authors
POZSGAY V TRINH L SHILOACH J ROBBINS JB DONOHUEROLFE A CALDERWOOD SB
Citation
V. Pozsgay et al., PURIFICATION OF SUBUNIT-B OF SHIGA TOXIN USING A SYNTHETIC TRISACCHARIDE-BASED AFFINITY MATRIX, Bioconjugate chemistry, 7(1), 1996, pp. 45-55
Citations number
47
Categorie Soggetti
Biology,Chemistry,"Biochemical Research Methods
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
7
Issue
1
Year of publication
1996
Pages
45 - 55
Database
ISI
SICI code
1043-1802(1996)7:1<45:POSOST>2.0.ZU;2-X
Abstract
The blood group P-1 antigenic trisaccharide (3), which is the receptor -binding ligand of Shiga-like toxins, is synthesized in a spacer-equip ped form (32) from 2-(trimethylsilyl)ethyl glucoside 5 and the 1-thiog alactoside building blocks 10 and 22 in a stereocontrolled, stepwise f ashion. Covalent attachment of 32 to hydrazine group-containing agaros e gel by reductive amination provided the P-1 trisaccharide-containing affinity sorbent which was used for preparative scale isolation of su bunit B of Shiga toxin.