DETERMINATION OF NICOTIAMIDE BY PHOTOCHEMICAL FLUOROMETRY

An on-line photochemical spectrofluorimetric method for the determinat ion of nicotinamide is proposed. It is based on the conversion of nico tinamide in an acidic medium into an intensively fluorescent compound. The calibration graph was linear up to 10.0 mu g ml(-1) nicotinamide (r = 0.999), the limit of detection was 0.60 ng ml(-1) nicotinamide an d the relative standard deviations were 1.5% (n = 6), 2.1% (n = 6)and 2.1% (n = 6)for 50 ng ml(-1), 0.70 mu g ml(-1) and 1.0 mu g ml(-1) nic otinamide, respectively. The kinetic behaviors of the reaction and the effects of some experimental conditions were investigated. Its applic ation to assay of vitamin B complex tablets was found to be satisfacto ry.