BRADYKININ ANTAGONISTS HOE-140 AND CP-0597 DIMINISH MICROCIRCULATORY INJURY AFTER ISCHEMIA-REPERFUSION OF THE PANCREAS IN RATS

Ischaemia-reperfusion of the pancreas was performed in 35' anaesthetiz ed Sprague-Dawley rats. The effects of two bradykinin antagonists, HOE -140 (13 mu g kg(-1) intravenous bolus injection, n = 7) and CP-0597 ( 18 mu g kg(-1) h(-1) intravenous infusion, n = 7) on pancreatic microv ascular perfusion and leucocyte-endothelium interaction were quantitat ively analysed by intravital fluorescence microscopy. Three further gr oups underwent sham operation (n = 7), ischaemia of 2 h without treatm ent (n = 7), and ischaemia of 2 h with infusion of phosphate buffer (n = 7). Functional capillary density was significantly greater in anima ls treated with HOE-140 or CP-0597 than in sham-treated animals, and w as decreased to only 60 per cent. Adherence of leucocytes to the endot helium of postcapillary venules was significantly reduced when compare d with ischaemia without antagonist. These results demonstrate a posit ive effect of the two bradykinin antagonists HOE-140 and CP-0597 on mi crovascular perfusion failure after ischaemia-reperfusion of the pancr eas.