TIME-DEPENDENT HISTAMINE-RELEASE FROM STORED HUMAN BLOOD PRODUCTS

Perioperative tranfusion of whole blood has been shown to amplify trau ma-induced immunosuppression, which could be attenuated by perioperati ve administration of histamine(2) receptor antagonists. Supernatants f rom different blood products were, therefore, analysed for histamine c ontent during storage. Whole blood (six units), plasma-reduced whole b lood (six units), and plasma- and buffy coat-reduced (saline-adenine-g lucose-mannitol) (SAGM) blood (six units) from unpaid healthy donors w ere stored in the blood bank for 35 days at 4 degrees C. Plasma histam ine and total cell-bound histamine content at donation, and histamine concentration in samples drawn from the units on days 0, 2, 5, 9, 14, 21, 28 and 35 were analysed with an enzyme-linked immunosorbent assay. Median plasma histamine concentration was 4.8 (range 1.9-14.3) nmol/l (n = 18). Median total cell-bound histamine content was 417.0 (range 176.0-910.0) nmol/l in whole blood and 475.0 (range 360.0-1560.0) nmol /l in plasma-reduced whole blood, while it was undetectable in SAGM bl ood. Spontaneous histamine release increased in a time-dependent manne r from a median of 6.7 (range 2.2-17.4) nmol/l at the time of storage to 175.0 (range 33.0-485.0) nmol/l at day 35 in whole blood, from 18.8 (range 8.2-38.5) to 328.5 (range 224.0-1137.0) nmol/l in plasma-reduc ed whole blood, and from 0.5 (range 0.5-1.5) to 2.2 (range 1.4-6.9) nm ol/l in SAGM blood. These results show spontaneous histamine release d uring storage of human blood products which contain full amount of leu cocytes, and this may play a significant role in detrimental effects o f blood transfusion.