Ej. Eisenberg et Wm. Eickhoff, DETERMINATION OF ETOPOSIDE IN BLOOD BY LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Journal of chromatography. Biomedical applications, 621(1), 1993, pp. 110-114
A liquid chromatographic (LC) method for determination of etoposide in
dog blood is described. The technique includes solvent extraction of
etoposide using a dichloroethane-hexane mixture and reconstitution of
the drug in an aqueous reconstitution solution. The samples are analyz
ed by reversed-phase LC with electrochemical detection. Validation of
the method demonstrated good sensitivity, precision and reproducibilit
y. The method is useful for the study of etoposide pharmacokinetics in
the dog.