PMPY-ZAP - A REUSABLE POLYMERASE CHAIN REACTION-DIRECTED GENE DISRUPTION CASSETTE FOR SACCHAROMYCES-CEREVISIAE

Authors
SCHNEIDER BL STEINER B SEUFERT W FUTCHER AB
Citation
Bl. Schneider et al., PMPY-ZAP - A REUSABLE POLYMERASE CHAIN REACTION-DIRECTED GENE DISRUPTION CASSETTE FOR SACCHAROMYCES-CEREVISIAE, Yeast, 12(2), 1996, pp. 129-134
Citations number
11
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology",Biology
Journal title
Yeast → ACNP
ISSN journal
0749503X
Volume
12
Issue
2
Year of publication
1996
Pages
129 - 134
Database
ISI
SICI code
0749-503X(1996)12:2<129:P-ARPC>2.0.ZU;2-0
Abstract
Gene disruption is an important method for genetic analysis in Sacchar omyces cerevisiae. We have designed a polymerase chain reaction-direct ed gene disruption cassette that allows rapid disruption of genes in S . cerevisiae without previously cloning them. In addition, this casset te allows recycling of URA3, generating gene disruptions without the p ermanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.