ANALYSIS OF A 36.2 KB DNA-SEQUENCE INCLUDING THE RIGHT TELOMERE OF CHROMOSOME-VI FROM SACCHAROMYCES-CEREVISIAE

The nucleotide sequence of a 36.2-kb distal region containing the righ t telomere of chromosome VI was determined. Both strands of DNA cloned into cosmid clone 9965 and plasmid clone pEL174P2 were sequenced with an average redundancy of 7.9 per base pair, by both dye primer and dy e terminator cycle sequencing methods. The G + C content of the sequen ce was found to be 37.9%. Eighteen open reading frames (ORFs) longer t han 100 amino acids were detected. Four of these ORFs (9965orfR017, 99 65orfF016, 9965orfR009 and 9965orfF003) were found to encode previousl y identified genes (YMR31, PRE4, NIN1 and HXK1, respectively). Six ORF s (9965orfR013, 9965orfF018, 9965orfF006, 9965orfR014, 9965orfF013 and 9965orfR020) were found to be homologous to hypothetical 121.4-kDa pr otein in the BCK 5' region, Bacillus subtilis DnaJ protein, hypothetic al Trp-Asp repeats containing protein in DBP3-MRPL27, putative mitocho ndrial carrier YBR291C protein, Salmonella typhimurium nicotinate-nucl eotide pyrophosphorylase, and Escherichia coli cystathionine beta-lyas e, respectively. The putative proteins encoded by 9965orfF018, 9965orf R014 and 9965orfR020 were found to be, respectively, a new member of t he family of DnaJ-like proteins, the mitochondrial carrier protein and cystathionine lyase. The nucleotide sequence reported here has been d eposited in the DDBJ/GenBank/EMBL data library under Accession Number D44597.