KAPPA-MARKER TYPING WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - IDENTIFICATION OF KAPPA-MARKER SPECIFIC TRYPTIC PEPTIDE FROM THE KAPPA-LIGHT-CHAIN OF IMMUNOGLOBULIN-G
R. Iida et al., KAPPA-MARKER TYPING WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - IDENTIFICATION OF KAPPA-MARKER SPECIFIC TRYPTIC PEPTIDE FROM THE KAPPA-LIGHT-CHAIN OF IMMUNOGLOBULIN-G, Journal of chromatography. Biomedical applications, 622(1), 1993, pp. 9-12
Human serum immunoglobulin G was separated into its heavy and light ch
ains by sodium dodecyl sulfate polyacrylamide gel electrophoresis and
transferred electrophoretically to a polyvinylidenedifluoride membrane
. Peptide fragments liberated from the light chain by in situ digestio
n with trypsin were then analyzed by reversed-phase high-performance l
iquid chromatography (HPLC). On comparing the HPLC patterns of these f
ragments derived from three major kappa marker (Km) types, two distinc
t peaks specific for the Km types were detected. Sequencing of the two
specific peak peptides confirmed that they were identical to a stretc
h comprising residues 191-207 of the immunoglobulin kappa light chain,
which contains valine/leucine allotypic variation at position 191.