DETERMINATION OF TRIS(HYDROXYMETHYL)AMINOMETHANE (TROMETHAMINE) IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

Authors
MORRIS MJ HSIEH JYK
Citation
Mj. Morris et Jyk. Hsieh, DETERMINATION OF TRIS(HYDROXYMETHYL)AMINOMETHANE (TROMETHAMINE) IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION, Journal of chromatography. Biomedical applications, 622(1), 1993, pp. 87-92
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
622
Issue
1
Year of publication
1993
Pages
87 - 92
Database
ISI
SICI code
0378-4347(1993)622:1<87:DOT(IH>2.0.ZU;2-Y
Abstract
Determination of tromethamine (2-amino-2-hydroxymethyl-1,3-propanediol ) in human plasma and urine has been developed and validated. The meth od utilizes reversed-phase high-performance liquid chromatographic sep aration and fluorescence detection of a pre-column derivative. The ass ay was linear in the ranges of 1-200 mu g/ml plasma and 5-500 mu g/ml urine with accuracies (mean percent recovery) all within 10% of 100% a nd precisions (coefficient of variation) all less than 10%.