HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DROTAVERINE IN HUMAN PLASMA AND URINE

Citation
Oo. Bolaji et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DROTAVERINE IN HUMAN PLASMA AND URINE, Journal of chromatography. Biomedical applications, 622(1), 1993, pp. 93-97
Citations number
9
Categorie Soggetti
Chemistry Analytical
ISSN journal
03784347
Volume
622
Issue
1
Year of publication
1993
Pages
93 - 97
Database
ISI
SICI code
0378-4347(1993)622:1<93:HLMFTD>2.0.ZU;2-0
Abstract
A simple and sensitive HPLC method for the determination of drotaverin e in human plasma and urine has been developed. Alkalinized plasma or urine was extracted with organic solvent and the basic components in t he organic phase were back-extracted into 0.1 M HCl. An aliquot of the aqueous layer was injected onto the column and the eluent was monitor ed at 254 nm. Separation was performed on a C-18-column with 0.02 M so dium dihydrogen phosphate-methanol (30:70, v/v) containing perchlorate ion at pH 3.2 as mobile phase. Drotaverine was well resolved from the plasma constituents and internal standard. An excellent linearity was observed between peak-height ratios and plasma concentrations and the intra- and inter-assay coefficients of variation were always <10%. Th e lowest limit of detection (signal-to-noise ratio 3:1) was 6 ng/ml. T he method is suitable for therapeutic monitoring and pharmacokinetic s tudies of drotaverine in humans as well as in animal models.