Oo. Bolaji et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DROTAVERINE IN HUMAN PLASMA AND URINE, Journal of chromatography. Biomedical applications, 622(1), 1993, pp. 93-97
A simple and sensitive HPLC method for the determination of drotaverin
e in human plasma and urine has been developed. Alkalinized plasma or
urine was extracted with organic solvent and the basic components in t
he organic phase were back-extracted into 0.1 M HCl. An aliquot of the
aqueous layer was injected onto the column and the eluent was monitor
ed at 254 nm. Separation was performed on a C-18-column with 0.02 M so
dium dihydrogen phosphate-methanol (30:70, v/v) containing perchlorate
ion at pH 3.2 as mobile phase. Drotaverine was well resolved from the
plasma constituents and internal standard. An excellent linearity was
observed between peak-height ratios and plasma concentrations and the
intra- and inter-assay coefficients of variation were always <10%. Th
e lowest limit of detection (signal-to-noise ratio 3:1) was 6 ng/ml. T
he method is suitable for therapeutic monitoring and pharmacokinetic s
tudies of drotaverine in humans as well as in animal models.