Aka. Mandal et al., DIRECT SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION FROM COTYLEDONARY LEAVES OF SAFFLOWER, Plant cell, tissue and organ culture, 43(3), 1995, pp. 287-289
Somatic embryos were induced directly on adaxial surface of cotyledona
ry leaves within 8-10 days of culture on Murashige and Skoog medium co
ntaining 5.37 to 10.74 mu M 1 - napthaleneacetic acid and 2.22 mu M be
nzyl adenine. Germinated embryos with shoot axes developed into comple
te plants after transfer onto half stength Murashige and Skoog medium
containing 1.07 mu M 1 - napthaleneacetic acid. Histological studies s
uggested direct origin of somatic embryos with broad-base attachment.