U. Kreutzer et T. Jue, H-1-NMR SIGNAL OF ARENICOLA-MARINA MYOGLOBIN IN-VIVO AS AN INDEX OF TISSUE OXYGENATION, European journal of biochemistry, 235(3), 1996, pp. 622-628
Key questions on how intertidal animals adapt to hypoxic stress center
on the high energy phosphate response to decreasing oxygenation. With
recent H-1/P-31-NMR techniques to monitor mammalian tissue metabolism
, a novel approach has emerged to observe potentially the intracellula
r oxygen interaction in invertebrates. The present study indicates tha
t Arenicola marina, a standard model for intertidal animals, exhibits
a distinct set of Mb H-1-NMR signals ill vivo, corresponding to the tw
o isolated Mb isoforms. Specifically both deoxy-Mb I and deoxy-Mb II e
xhibit paramagnetically shifted signals at 93.4 ppm and 92.5 ppm at 25
degrees C, respectively, which arise from the proximal histidyl Ndelt
aH. These signals reflect the cellular oxygenation slate and indicate
clearly that the phosphotaurocyamine level begins to drop al the onset
of anoxia and declines gradually to 50% of control after 3.5 h. H-1 M
b spectra indicate protein heterogeneity originating from heme as well
as structural disorder.