Jw. Cherwonogrodzky, COMPARISON OF RABBIT MONOSPECIFIC ANTIBRUCELLA ANTISERA WITH MOUSE ANTIBRUCELLA MONOCLONAL-ANTIBODIES, Archivos de medicina veterinaria, 27, 1995, pp. 131-137
Brucella abortus biovars 1-7 and 9 were agglutinated with dilutions of
rabbit monospecific antisera (cross-absorbed and specific either for
''A'' or ''M'' antigens) or mouse monoclonal antibodies (not cross-abs
orbed, YsT9-3 with ''A'' specificity for B. abortus 1119-3 O-polysacch
aride [OPS], and Bm-15 with ''M'' specificity for B. melitensis 16M OP
S). Observed similarities were that both rabbit anti-''A'' antiserum a
nd mouse monoclonal YsT9-3 antibody agglutinated biovars 1-3 and 6 but
not biovars 4, 5 and 9. Also, rabbit anti-''M'' antiserum agglutinate
d only biovars 4, 5, 7 and 9 while mouse monoclonal Bm-15 antibody, wh
ich at the higher concentrations it agglutinated the other biovars, at
the lower concentrations it agglutinated these same biovars. Differen
ces, aside from the stronger agglutination reactions for the monoclona
l antibodies, were that rabbit anti-''A'' antiserum recognized an ''A'
' antigen on B. abortus biovar 7 whereas mouse monoclonal YsT9-3 antib
ody did not. Also, as noted previously, high concentrations of mouse m
onoclonal Bm-15 antibody agglutinated B. abortus ''A'' biovars 1, 2, 3
and 6 whereas rabbit anti-''M'' antisera did not.