MICROWAVE EXTRACTION OF INCURRED SALINOMYCIN FROM CHICKEN TISSUES

A rapid, accurate, environmentally friendly and cost-effective microwa ve extraction technique was developed for the extraction of spiked and incurred salinomycin from chicken tissues (kidney, liver, muscle, ova rian yolk and fat). Extraction of salinomycin from various tissues was achieved by irradiating the sample in absolute ethanol and 2-propanol (15+2) for 9 sec. in a common household microwave oven. The extract w as analysed without further cleanup by HPLC on a C-18 column (5 mu m) and detected at 592 nm via post-column reaction with 4-dimethylaminobe nzaldehyde (DMABA) in a heated reactor coil at 86 degrees C. Recoverie s of salinomycin from spiked tissues at 30 ng/g level ranged between 8 7 and 100%. The limit of quantitation was found to be 10 ng/g. The dev eloped method was applied for the analysis of incurred tissues and ova rian yolk of laying chickens given sodium salinomycin in feed at diffe rent levels for 14 consecutive days followed by withdrawal periods. Re sidues were detected in all tissues and ovarian yolk at 0 withdrawal t ime but declined during the withdrawal period. Highest residue were fo und in fat and ovarian yolk.