IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE HIGH-AFFINITY CHOLINE CARRIER FROM RAT-BRAIN STRIATUM

Authors
RYLETT RJ WALTERS SA DAVIS W
Citation
Rj. Rylett et al., IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE HIGH-AFFINITY CHOLINE CARRIER FROM RAT-BRAIN STRIATUM, Molecular brain research, 35(1-2), 1996, pp. 354-358
Citations number
21
Categorie Soggetti
Neurosciences
Journal title
Molecular brain research → ACNP
ISSN journal
0169328X
Volume
35
Issue
1-2
Year of publication
1996
Pages
354 - 358
Database
ISI
SICI code
0169-328X(1996)35:1-2<354:IAPCOT>2.0.ZU;2-K
Abstract
[H-3]Choline mustard aziridinium ion binds irreversibly to the sodium- coupled high-affinity choline transport protein in a sodium-dependent and hemicholinium-sensitive manner, and thus is a useful affinity liga nd. In rat striatal synaptosomal membranes, it radiolabels two polypep tides with apparent molecular masses of 58 and 35 kDa. Based upon the use of two different experimental approaches, it appears that neither of these polypeptides is glycosylated.