A. Jarry et al., DIRECT SECRETORY EFFECT OF INTERLEUKIN-1 VIA TYPE-I RECEPTORS IN HUMAN COLONIC MUCOUS EPITHELIAL-CELLS (HT29-CL.16E), Gut, 38(2), 1996, pp. 240-242
The stable differentiated human colonic epithelial cell line, HT29-Cl.
16E, was used to study the effects of interleukin-1 (IL-1) on mucin ex
ocytosis. The main findings include: (a) IL-1 stimulated a rapid relea
se of mucin from filter grown HT29-Cl.16E cells, this effect being dos
e related; (b) this secretory effect was abolished in the presence of
the blocking monoclonal antibody M4 specific for IL-1 receptors type I
, showing that IL-1 receptors type I mediated IL-1 action; (c) experim
ents based on chamber cultures showed that these receptors were locate
d on the basolateral membranes of HT29-Cl.16E cells; (d) finally, mRNA
for IL-1 receptors type I were detected by reverse transcriptase-poly
merase chain reaction in these cells. To extend these findings to the
in vivo situation, the rapid stimulatory effect of IL-1 on mucin exocy
tosis may contribute to the wash out of noxious agents during mucosal
inflammation.