Me. Azam et al., ESTIMATION OF AZADIRACHTIN-A CONTENT OF EMULSIFIABLE AND SOLUTION CONCENTRATES OF NEEM, Journal of AOAC International, 78(4), 1995, pp. 893-896
A column chromatographic cleanup method to remove aliphatic and aromat
ic solvent components and different surfactants present in formulated
neem was developed. A glass column containing silica gel (60-120 Briti
sh Standard Sieve) and 250 mL hexane-acetone (6 + 4) was used. Impurit
ies interfering in the region of the azadirachtin-A peak were removed
by fractional elution. Recovery of azadirachtin-A was greater than 81%
.