ANALYSIS OF POLYCHLORINATED-BIPHENYLS IN FATTY BIOLOGICAL MATRICES BYONLINE SUPERCRITICAL-FLUID EXTRACTION AND SUPERCRITICAL-FLUID CLEANUP

We present a method that combines the extraction of polychlorinated bi phenyls (PCBs) and their separation from relatively large quantities o f fat in biological matrixes by combined supercritical fluid extractio n and separation, Cyano-functionalized silica gel, silica gel, aluminu m oxide, Florisil, 3-aminopropyl-functionalized silica gel, and octade cyl-functionalized silica gel were tested for suitability as chromatog raphic media for separation of PCBs from lipids, Silica gel, 3-aminopr opyl-functionalized silica gel, and Florisil adequately separated PCBs from lipids when eluted with supercritical CO2, Florisil allowed both the extraction of PCBs and their separation from lipids in PCB-spiked chicken egg and fish, Two grams of sample containing PCBs at 0.125 mu g/g was sufficient for subsequent separations and the low-level analy sis required for the more toxic PCB components.