CLONING AND HETEROLOGOUS EXPRESSION OF THE ENTIRE SET OF STRUCTURAL GENES FOR NIKKOMYCIN SYNTHESIS FROM STREPTOMYCES-TENDAE TU901 IN STREPTOMYCES-LIVIDANS

Authors
BORMANN C MOHRLE V BRUNTNER C
Citation
C. Bormann et al., CLONING AND HETEROLOGOUS EXPRESSION OF THE ENTIRE SET OF STRUCTURAL GENES FOR NIKKOMYCIN SYNTHESIS FROM STREPTOMYCES-TENDAE TU901 IN STREPTOMYCES-LIVIDANS, Journal of bacteriology, 178(4), 1996, pp. 1216-1218
Citations number
19
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
178
Issue
4
Year of publication
1996
Pages
1216 - 1218
Database
ISI
SICI code
0021-9193(1996)178:4<1216:CAHEOT>2.0.ZU;2-4
Abstract
A genomic library from Streptomyces tendae raised in shuttle cosmid ve ctor pKC505 was screened with a previously isolated 8-kb DNA fragment containing the orfP1 gene, which is involved in nikkomycin biosynthesi s. The entire set of structural genes for nikkomycin synthesis was het erologously expressed in S. lividans TK23 by introducing recombinant c osmids p24/32 and p9/43-2, carrying inserts of about 31 and 27 kb, res pectively, overlapping by 15 kb. S. lividans transformants synthesized nikkomycins X, Z, I, and J, which mere identified by high-pressure li quid chromatography analyses of culture filtrates.