REDUCED ENZYMATIC ANTIOXIDANT DEFENSE-MECHANISM IN KIDNEY TISSUES FROM GENTAMICIN-TREATED GUINEA-PIGS - EFFECTS OF VITAMIN-E AND VITAMIN-C

Citation
M. Kavutcu et al., REDUCED ENZYMATIC ANTIOXIDANT DEFENSE-MECHANISM IN KIDNEY TISSUES FROM GENTAMICIN-TREATED GUINEA-PIGS - EFFECTS OF VITAMIN-E AND VITAMIN-C, Nephron, 72(2), 1996, pp. 269-274
Citations number
36
Categorie Soggetti
Urology & Nephrology
Journal title
ISSN journal
00282766
Volume
72
Issue
2
Year of publication
1996
Pages
269 - 274
Database
ISI
SICI code
0028-2766(1996)72:2<269:READIK>2.0.ZU;2-U
Abstract
In this study, the activities of major enzymes participating in free r adical metabolism (xanthine oxidase, XO; Cu,Zn and Mn superoxide dismu tases, SOD; glutathione peroxidase, GSH-Px; catalase, CAT) were measur ed in kidney tissues from guinea pigs treated with gentamicin alone (2 00 mg/kg/day), gentamicin plus vitamin C (600 mg/kg/day), gentamicin p lus vitamin E (400 mg/kg/day), and gentamicin plus vitamins C and E to gether for 10 days, and from animals treated with physiological saline solution alone during this period. We found no significant difference s between control and gentamicin groups with respect to XO and Cu,Zn-S OD activities. However, the activities of Mn-SOD, GSH-Px, and CAT were found to be significantly depressed in the gentamicin-treated group r elative to controls. In the gentamicin plus vitamin C group, the renal tissue Mn-SOD activity was found to be higher as compared with contro l and gentamicin groups. In this group, XO, GSH-Px and CAT activities were also higher than in the gentamicin-treated group, but no statisti cally significant differences existed between the values of this group and controls. Similar results were also observed in the gentamicin pl us vitamin E group for Mn-SOD, GSH-Px, CAT, and XO. In this group, the Cu,Zn-SOD activity was found to be decreased as compared with control and gentamicin groups. In the gentamicin plus vitamins C and E group, the Cu,Zn-SOD activity was found to be decreased, the XO activity to be unchanged, and Mn-SOD, GSH-Px, and CAT activities to be increased a s compared with the gentamicin and control groups. The results suggest that the enzymatic antioxidant defense system was significantly distu rbed because of the suppressed activities of Mn-SOD, GSH-Px, and CAT i n the kidney tissues from animals treated with gentamicin. However, vi tamins C and E given concurrently with gentamicin completely abrogated this enzymatic suppression.