FIBRINOGEN BINDING TO ICAM-1 ON EA.HY-926 ENDOTHELIAL-CELLS IS DEPENDENT ON AN INTACT CYTOSKELETON

Citation
A. Vandestolpe et al., FIBRINOGEN BINDING TO ICAM-1 ON EA.HY-926 ENDOTHELIAL-CELLS IS DEPENDENT ON AN INTACT CYTOSKELETON, Thrombosis and haemostasis, 75(1), 1996, pp. 182-189
Citations number
60
Categorie Soggetti
Hematology,"Cardiac & Cardiovascular System","Peripheal Vascular Diseas
Journal title
ISSN journal
03406245
Volume
75
Issue
1
Year of publication
1996
Pages
182 - 189
Database
ISI
SICI code
0340-6245(1996)75:1<182:FBTIOE>2.0.ZU;2-5
Abstract
Fibrinogen is a ligand for Intercellular Adhesion Molecule-1 (ICAM-1), and enhances monocyte-endothelial cell interaction by coupling Mac-1 on monocytes to ICAM-1 on endothelial cells. We investigated the role of the cytoskeleton in fibrinogen binding to the human endothelial cel l line EA.hy 926 using immunofluorescence techniques. In this cell lin e TNF alpha induced the simultaneous appearance of stress fibers and o f ICAM-1, which was clustered predominantly on endothelial cell projec tions. Incubation of TNF alpha-stimulated endothelial cells with fibri nogen resulted in binding of fibringen to ICAM-1 on these cell project ions. Disruption of the cytoskeleton by cytocholasin B abolished fibri nogen binding. Activation of protein kinase C with 12-O-tetradecanoyl phorbol-13-acetate resulted in simultaneous loss of both stress fibers and Fibrinogen binding. These results suggest that a connection betwe en ICAM-1 and the cytoskeleton results in clustering of ICAM-1 on cell projections, which is required for fibrinogen binding.