ELECTRONIC SPIN-RESONANCE QUANTITATIVE-ANALYSIS OF MN(II) COMPLEXES ANTIOXIDATIVE ACTIVITY USING PHOSPHATE OR ORGANIC BUFFER

Superoxide anion is implicated in the pathogenesis of several human di seases including ischaemia, atheroscelerosis and inflammation when mol ecular or enzymatic antioxidant systems cannot regulate its formation. In order to investigate superoxide dismutase (SOD) mimics, a method u sing Electron Spin Resonance (ESR) has been developed to quantify anti oxidative activities of Mn(II) compounds. The acetaldehyde / xanthine oxidase system produced superoxide anion and the 5,5-dimethy-1-pyrolin e-N-oxide (DMPO) was used as spin trap. The complexing properties of t he usual phosphate buffer towards Mn2+ being not negligible comparativ ely to those of the studied ligands, activities of Free ligands and Mn (II) complexes have been compared according to the buffer, phosphate o r organic.