Dry biomass obtained from stressed cells of Chlorella vulgaris (rich i
n carotenoid pigments) was used as such in animal feed, instead of the
commercial synthetic pigment. The in vivo effect of microalgal biomas
s as substitute pigment was ascertained with Hisex brown hens kept lay
ing during 37 days under conventional conditions, and strongly suggest
ed that yolk pigmentation was comparable to that obtained using commer
cial pigments, when comparable weight of colourant was formulated into
the feed.