M. Petrovic et al., THE DNA-BINDING AFFINITY OF RAT-LIVER NUCLEOPROTEINS TO THE REGULATORY ELEMENTS OF THE HAPTOGLOBIN AND ALPHA(2)-MACROGLOBULIN GENES, Cell biology international, 19(12), 1995, pp. 967-972
Transcriptional regulation and binding interactions between soluble nu
cleoproteins and the hormone response elements (REs) of the rat haptog
lobin (Hp) and alpha(2)-macroglobulin (alpha(2)MG) genes was examined
in the livers of rats during the acute-phase reaction. Our results dem
onstrate that the elevation of the Hp and alpha(2)MG genes' transcript
ion rates in acute-phase liver relies essentially on an increase in th
e binding-affinity of pre-xisting soluble nucleoproteins, enhancing th
eir capability to bind the examined cis-regulatory elements. The 35kD
nucleoprotein that displayed an acute-phase inducible affinity to bind
hormone REs of rat Hp and (alpha(2)MG genes, was identified as a C/EB
P beta isoform.