NEW FLUORESCENT CALCIUM INDICATORS DESIGNED FOR CYTOSOLIC RETENTION OR MEASURING CALCIUM NEAR MEMBRANES

Citation
C. Vorndran et al., NEW FLUORESCENT CALCIUM INDICATORS DESIGNED FOR CYTOSOLIC RETENTION OR MEASURING CALCIUM NEAR MEMBRANES, Biophysical journal, 69(5), 1995, pp. 2112-2124
Citations number
32
Categorie Soggetti
Biophysics
Journal title
ISSN journal
00063495
Volume
69
Issue
5
Year of publication
1995
Pages
2112 - 2124
Database
ISI
SICI code
0006-3495(1995)69:5<2112:NFCIDF>2.0.ZU;2-I
Abstract
A new family of fluorescent calcium indicators has been developed base d on a new analog of BAPTA called FF6. This new BAPTA analog serves as a versatile synthetic intermediate for developing Ca2+ indicators tar geted to specific intracellular environments. Two of these new Ca2+ in dicators, fura-PE3 and fura-FFP18, are described in this report. Fura- PE3 is a zwitterionic indicator that resists the rapid leakage and com partmentalization seen with fura-2 and other polycarboxylate calcium i ndicators. In contrast to results obtained with fura-2, cells loaded w ith PE3 remain brightly loaded and responsive to changes in concentrat ion of cytosolic free calcium for hours. Fura-FFP18 is an amphipathic indicator that to binds to liposomes and to cell membranes. Studies to be detailed later indicate that FFP18 functions as a near-membrane Ca 2+ indicator and that calcium levels near the plasma membrane rise fas ter and higher than in the cytosol.