DETECTION OF INFECTIOUS BOVINE-RHINOTRACH EITIS VIRUS BY HYBRIDIZATION WITH NONRADIOACTIVE DNA PROBES

Biotin-labeled DNA probes for infectious bovine rhinotracheitis virus also known as bovine herpesvirus-1 (BHV-1) have been developed. nle pr ocedure: is based on dot-blot hybridization using biotin-labeled bacte riophage M13 and plasmid probes containing cloned PstI and EcoRI-PstI restriction fragments of viral genome, Tile probes obtained were used to detect viral nucleic acids in specimens of bovine spermatic fluid o r nasal swabs of calves. The method is simple and rapid, taking less t han 24 h, and is highly specific and sensitive, this recommending it f or practical veterinary.