J. Shipley et al., INTERPHASE FLUORESCENCE IN-SITU HYBRIDIZATION AND REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION AS A DIAGNOSTIC-AID FOR SYNOVIAL SARCOMA, The American journal of pathology, 148(2), 1996, pp. 559-567
Identification of the t(X;18)(p11.2;q11.2) that is associated with a h
igh proportion of synovial sarcoma can be a useful diagnostic aid. The
translocation results in fusion of the SYT gene on chromosome 18 to e
ither the SSX1 or the SSX2 gene, two homologous genes within Xp11.2 Tw
o-color interphase fluorescence in situ hybridzation and reverse trans
cription polymerase chain reaction were assessed as approaches to iden
tify the rearrangement in well characterized cases. The presence of th
e translocation, and the specific chromosome X gene disrupted, were in
ferred from the configuration of signals from chromosome-specific cent
romere probes, paints, and markers flanking each gene in preparations
of interphase nuclei. Rearrangement was found in two cell lines and ei
ght of nine tumor samples, including analysis of five touch imprints.
This was consistent with cytogenetic data in four cases and reverse tr
anscription polymerase chain reaction analysis using primers known to
amplify both SYT-SSX1 and SYT-SSX2 transcripts. The transcripts were d
istinguished by restriction with LspI and SmaI. Contrary to previous s
uggestions, there was no obvious correlation between histological subt
ype and involvement of the SSX1 or SSX2 gene. These approaches could a
lso be applied to the identification of tumor-free margins and metasta
tic disease.