STRONG EXPRESSION OF PEROXISOMAL MALATE SYNTHASE GENES FROM N-ALKANE-UTILIZABLE YEAST CANDIDA-TROPICALIS IN SACCHAROMYCES-CEREVISIAE

Expression of the genes encoding several peroxisomal enzymes of the n- alkane-utilizable yeast Candida tropicalis has been examined in Saccha romyces cerevisiae to find out a novel and foreign promoter functional in S. cerevisiae. Among these genes, two malate synthase genes, as we b as the isocitrate lyase gene, were strongly expressed in acetate-gro wn cells of S. cerevisiae. The amounts of the recombinant malate synth ases expressed in S. cerevisiae were as much as 30% of the total amoun t of soluble proteins in the cells. These results showed that the upst ream regions (1.1 kbp for the MS-1 gene and 0.8 kbp for the MS-2 gene) of the malate synthase genes [UPR-MSs] from C. tropicalis contained s trong promoters functional in S. cerevisiae and that the construction of novel gene expression systems-using UPR-MSs might be possible.