STUDY OF MAGNETIC-FIELD EFFECTS ON FIBROBLAST-CULTURES .1. THE EVALUATION OF THE EFFECTS OF STATIC AND EXTREMELY-LOW-FREQUENCY (ELF) MAGNETIC-FIELDS ON VITAL FUNCTIONS OF FIBROBLASTS
B. Kula et M. Drozdz, STUDY OF MAGNETIC-FIELD EFFECTS ON FIBROBLAST-CULTURES .1. THE EVALUATION OF THE EFFECTS OF STATIC AND EXTREMELY-LOW-FREQUENCY (ELF) MAGNETIC-FIELDS ON VITAL FUNCTIONS OF FIBROBLASTS, Bioelectrochemistry and bioenergetics, 39(1), 1996, pp. 21-26
Cultured fibroblasts isolated from murine livers by tissue trypsinizat
ion were exposed to a static magnetic field (0.49 T) and an extremely
low frequency (ELF) magnetic field (50 Hz, 0.020 T). The cultures were
exposed to magnetic fields on four consecutive days for exposure time
s of 2, 4, 8, 16, 32 and 64 min. After exposure the following paramete
rs of the fibroblast cultures were determined: the dynamics of culture
growth, the protein content and C-14-thymidine incorporation. The cyt
ometric parameters of the fibroblasts were also assessed. The ELF magn
etic field compromised vital functions of the fibroblasts (inhibition
of culture growth, decreased cellular protein, lowered cytometric para
meters) and caused a slowdown in the rate of C-14-thymidine incorporat
ion which indicates altered DNA synthesis. Ongoing experiments with a
static magnetic field have shown no effect on the vital functions of f
ibroblasts.