BEHAVIOR OF BETA(2)-MICROGLOBULIN IN THE ELECTRICAL DOUBLE-LAYER OF THE MERCURY-VERTICAL-BAR-SOLUTION INTERFACE

beta(2)-Microglobulin (beta(2)-m) is a small globular protein (12000 D a) which exists as a monomer in numerous body fluids. Data published o n the electrochemical behaviour of beta(2)-m are scarce. In this paper an attempt was made to get more information on the structure of the d ouble layer at mercury/solution interface in the presence of beta(2)-m . Using phase selective ac polarography the capacitive current-potenti al (C-E) curve was analysed as the function of various beta(2)-m conce ntration, pH of medium, temperature, denaturation agent concentration etc. The data obtained are discussed particularly from the aspect of f olding/unfolding state of the protein studied.